Extracting high molecular weight genomic DNA from Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Molecular cloning and sequencing of genomic DNA encoding aminopeptidase I from Saccharomyces cerevisiae.
Yeast aminopeptidase I is a vacuolar enzyme, which catalyzes the removal of amino acids from the NH2 terminus of peptides and proteins (Frey, J., and Rohm, K-H. (1978) Biochim. Biophys. Acta 527, 31-41). A yeast genomic DNA encoding aminopeptidase I was cloned from a yeast EMBL3A library and sequenced. The DNA sequence encodes a precursor protein containing 514 amino acid residues. The "mature"...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2018
ISSN: 2043-0116
DOI: 10.1038/protex.2018.076